Personale Docente

Mauro Dacasto

Professore ordinario

VET/07

Indirizzo: VIALE DELL'UNIVERSITÀ, 16 - LEGNARO PD . . .

Telefono: 0498272935

Fax: 0498272604

E-mail: mauro.dacasto@unipd.it

  • presso People can meet Prof. Dacasto at his home office (Agripolis Legnaro, Dept. of Comparative Biomedicine and Food Science, "Museo" building, first floor)
    It would be far better to make an appointment with Prof. Dacasto (tel. +39.049.827.2935; email: mauro.dacasto@unipd.it)

Research Topics

a. Comparative in vitro and in vivo studies on the expression and regulation of drug metabolizing enzymes (P450-dependent monooxygenases, conjugative enzymes, drug transporters).
In particular:
a.1. importance of constitutive factors, e.g. sex, age, species, breed/strain, individual susceptibility (pharmacogenetics);
a.2. identification of P450 isoforms responsible for the oxidative metabolism of different chemotherapeutics and contaminants;
a.3. effects of the exposure to the above cited substances, as well as to illegal growth promoters, on the expression and regulation of biotransformative enzymes (i.e. induction/inhibition phenomena).

b. Studies on xenobiotic molecular and cellular toxicity.
In this field of research, the following studies are likely to be recognised:
b.1. xenobiotic cytotoxicity testing;
b.2. in vitro studies on biochemical and molecular mechanisms of action of xenobiotics (i.e. apoptosis/necrosis) executed on target or surrogate tissues (i.e. the liver, the immune system).

c. Veterinary oncology and anti-cancer chemotherapy
c.1. transcriptomics of companion animal neoplasms
c.2. patient geno- and phenotyping (pharmacogenetics);
c.3. in vitro/in vivo studies on new therapeutic targets and characterization of their bio-molecular effects

d. Toxicogenomic studies, including:
d.1. studies concerning the effects of xenobiotics on the transcriptome of veterinary species;
d.2. studies devoted to the definition of biochemical/molecular biomarkers of internal dose or effect to be used in the screening approach.

Methods and techniques

1. Biochemical techniques (subcellular fractions isolation, enzymatic activities, immunoblotting)
2. Cell biology techniques (cultures and transfection of established cell lines, primary cell cultures, cytotoxicity testing, enzymatic activity in culture)
3. Molecular biology techniques (DNA/RNA isolation, PCR end point, Real time PCR, cloning, sequencing, cDNA microarray, RNA-seq).


Research Unit Staff

Principal Investigator
Mauro DACASTO (Full Prof., DVM, PhD, Dipl. ECVPT)
Members
Silvia DA ROS (MSc, PhD, post-doc)
Ramy ELGENDY (DVM, PhD student)
Mery GIANTIN (Associate Prof., DVM, PhD)
Rosa Maria LOPPARELLI (MSc, PhD, Technician)
Roberta MERLANTI (Assistant Prof., DVM, PhD, limited to research topic a)
Roberta TOLOSI (MSc, contract person)
Eleonora ZORZAN (MSc, PhD, post-doc)

PROPOSED DEGREE THESES OR INTERNSHIPS

Topic
Subjects of either degree theses or internships are linked to Proff. Dacasto and Giantin’s research topics.
In particular:
• Comparative in vitro and in vivo studies on the expression and regulation of drug metabolizing enzymes
• Studies on xenobiotic molecular and cellular toxicity
• Veterinary oncology and anticancer chemotherapy
• Pharmacogenetic and toxicogenomic studies

Length
From 6 months up to 2 years

Vacancy
Not defined

Requirements
• availability to attend either the pharmacogenetic/toxicogenomic or the cellular biology laboratories of the Division of Veterinary pharmaco-toxicology research unit
• interest for basic and applied pharmaco-toxicological sciences

Methodologies the student might learn
Molecular (including –omics) and cellular biology techniques

For further information, send an email to Prof. Dacasto (mauro.dacasto@unipd.it) or contact him by phone (+39.049.827.2935)